![]() ![]() To recap, read the case study a few times, watch the cohort definitely, and just double check everything asked on the tip sheet and rubric is in each task submitted. Task 3 seems overwhelming but you can literally copy parts of Task 1 and Task 2 and paste it into your presenter notes.just change the wording to reflect like you are speaking to the group of employees at EZ-Pleeze. For task one if you do not know what the role of each position is, just google it.ĭo the same thing for Task 2. Then went to the rubric grading in course tips and made sure everything listed under "competent" was in my essay. I wrote my essay following the tip sheet. I did not worry about page length, just include all that is asked and you will be good to go. You can literally outline your essays exactly how the tip sheets are laid out. I used the tip sheets.you need to use the tip sheets. Read case study again, and wrote down things I wanted to include in my essay as I read. Watch first section of cohort, took notes on what was needed. None of mine were sent back for revisions. I found though, that if I submit on Saturday, it was usually graded by Sunday lunch time. Then waited the week for it to be graded. ![]() I took 3 weeks but only worked on this class a total of 3 days. Watch the cohort - it has each task broken out and tells you EXACTLY what you need to do and even gives hints. The eukaryotic expression vector pEGFP-TASK3 is successfully constructed and the cell line stably expressing TASK3-eGFP fusion is established which is important for their fundamental research and potential applications.Ok so I was intimidated by this class due to reading a few posts on here and then seeing all the task requirements but let me start by saying DON'T BE. Compared with wild type SH-SY5Y cells, the cell viability of stable SH-SYSY-GFP tag-TASK3 cell line increased significantly with the same pH media, and the difference was statistically significant (P < 0.05). Exposure of the wild type SH-SY5Y cells and the stable SH-SY5Y-GFP tag-TASK3 cell line to different pH media (7.0, 6.7, 6.4, 6.1) for 24 h, the cell viability of two group cells significantly reduced with pH declining, and the difference was statistically significant (P < 0.05). The stable SH-SY5Y cell line expressing EGFP tagged-TASK3 fusion protein was successfully established. Exposure of the SH-SY5Y cell line expressing stably TASK3-eGFP fusion proteins was exposed to different pH media (7.0, 6.7, 6.4, 6.1) for 24 h, the cell viability was assessed with cell counting Kit-8 (CCK-8).Īll the results of identification by PCR, digestion with restriction endonuclease and sequencing indicated that the recombinant eukaryotic expression plasmid pEGFP-TASK3 was constructed correctly. The expression and localization of the EGFP tagged-TASK3 fusion protein was detected by Western blot and confocal microscope. The cell line stably expressiing EGFP tagged-TASK3 gene was established by screening with antibiotic G418 and fluorescence microscope. The correct recombinant expressing plasmid was transfected with X-feet transfection reagent to SH-SY5Y cells. TASK3 coding region was subcloned into pEGFP-N1 plasmid to construct a recombinant vector alled pEGFP-TASK3. ![]() To construct the acid-sensitive potassium hannel-3(TASK3) eukaryotic expression plasmid and to establish a stable SH-SY5Y cell line expressing enhanced green fluorescent protein (EGFP)-tagged TASK3. ![]()
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